Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Self-interference fluorescence microscopy with three-phase detection for depth-resolved confocal epi-fluorescence

Boy Braaf, Johannes F de Boer

    Optics Express
    |April 7, 2017
    PubMed
    Summary

    This study introduces self-interference fluorescence microscopy (SIFM) for faster 3D imaging of tissues. The novel method enables instantaneous depth-sensing, overcoming limitations of traditional confocal microscopy for in vivo applications.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Morphological artifacts in pulmonary pathology and literature review.

    Virchows Archiv : an international journal of pathology·2026
    Same author

    Hybrid particle-wave Monte Carlo OCT simulation method provides a three orders of magnitude improvement in efficiency.

    Biomedical optics express·2026
    Same author

    Comparison of Event-based Analysis Versus Trend-based Analysis in the Detection of Glaucoma Progression by Optical Coherence Tomography 3-Dimensional Rim Measurements.

    Journal of glaucoma·2025
    Same author

    Chromatic dispersion based axial length estimation using retinal spectral domain optical coherence tomography.

    Biomedical optics express·2025
    Same author

    Class of nonlinear filtering and windowing methods for image processing and reconstruction.

    Optics letters·2025
    Same author

    Ex vivo optical coherence tomography combined with near infrared targeted fluorescence: towards <i>in-vivo</i> esophageal cancer detection.

    Biomedical optics express·2024

    Area of Science:

    • Biophotonics
    • Microscopy
    • Optical Imaging

    Background:

    • 3D confocal fluorescence imaging of live tissues is hindered by sample movement and slow acquisition speeds.
    • Existing methods struggle to provide rapid, high-resolution depth information in dynamic biological samples.

    Purpose of the Study:

    • To develop a novel scanning confocal epi-fluorescence microscopy technique with instantaneous depth-sensing capabilities.
    • To overcome the limitations of current 3D imaging methods for in vivo and dynamic biological samples.

    Main Methods:

    • A tabletop epi-fluorescence setup utilizing self-interference fluorescence microscopy (SIFM) was engineered.
    • An annular phase plate generated a spectral self-interference signal phase-dependent on axial sample position.
    • A Mach-Zehnder interferometer with photon counters analyzed the SIFM signal for direct axial position encoding.

    Related Experiment Videos

    Main Results:

    • SIFM demonstrated a linear phase-depth relationship over 500 µm and 80 µm axial ranges for 4x and 10x objectives, respectively.
    • SIFM depth measurements showed strong agreement with optical coherence tomography.
    • High-resolution SIFM imaging was successfully applied to fluorescence angiography and ex vivo fly eye autofluorescence.

    Conclusions:

    • SIFM provides a robust method for instantaneous depth-sensing in fluorescence microscopy.
    • This technique significantly enhances 3D imaging capabilities for dynamic biological samples.
    • SIFM holds promise for advanced applications in fluorescence angiography and in vivo tissue imaging.