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Related Experiment Videos

Nanodiamonds as multi-purpose labels for microscopy.

S R Hemelaar1, P de Boer2, M Chipaux1

  • 1Groningen University, University Medical Center Groningen, Department of Biomedical Engineering, Antonius Deusinglaan 1, 9713, AW, Groningen, The Netherlands.

Scientific Reports
|April 9, 2017
PubMed
Summary

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This summary is machine-generated.

Fluorescent nanodiamonds with nitrogen-vacancy centers are versatile probes for advanced microscopy. They enable molecular targeting, internalization, and correlative imaging across fluorescence, electron, and magnetic techniques.

Area of Science:

  • Materials Science
  • Biotechnology
  • Nanotechnology

Background:

  • Nitrogen-vacancy (NV) centers in nanodiamonds offer unique photophysical properties for biological imaging.
  • Applications include fluorescence microscopy, cathodoluminescence (CL), and nanoscale sensing.
  • Versatile molecular labeling is crucial for utilizing small nanodiamonds in biological systems.

Purpose of the Study:

  • To demonstrate direct molecular targeting and internalization of nanodiamonds for biological applications.
  • To assess the suitability of nanodiamonds for correlative light and electron microscopy (CLEM).
  • To explore the potential of nanodiamonds for multimodal imaging and sensing.

Main Methods:

  • Streptavidin conjugation and antibody labeling for targeting biological molecules with 70 nm nanodiamonds.

Related Experiment Videos

  • Internalization studies using 40 nm nanodiamonds.
  • Osmium-fixation, plastic embedding, cathodoluminescence (CL), secondary electron (SE) imaging, and magnetic read-out.

    • Main Results:

    • Successful direct targeting of biological molecules and internalization of nanodiamonds.
    • Nanodiamond fluorescence is preserved after fixation and embedding, enabling CLEM.
    • CL and enhanced SE signals observed from embedded nanodiamonds.
    • Magnetic read-out demonstrated using pre-embedding fluorescence.

    Conclusions:

    • Nanodiamonds with NV centers are established as versatile probes for multimodal microscopy.
    • They facilitate correlation between fluorescence imaging, magnetometry, and ultrastructural electron microscopy.
    • This work highlights nanodiamonds as powerful tools for advanced biological investigations.