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Finally, Archaea Get Their CRISPR-Cas Toolbox
Uri Gophna1, Thorsten Allers2, Anita Marchfelder3
1Department of Molecular Microbiology and Biotechnology, George S. Wise Faculty of Life Sciences, Tel Aviv University, Tel Aviv 69978-01, Israel.
Trends in Microbiology
|April 11, 2017
Abstract:
The majority of archaea encode CRISPR-Cas systems but only a few CRISPR-Cas-based genetic tools have been developed for organisms from this domain. Nayak and Metcalf have harnessed a bacterial Cas9 protein for genome editing in Methanosarcina acetivorans, enabling efficient gene deletion and replacement.