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Related Experiment Videos

Intracellular pH and cell adhesion to solid substrate.

L B Margolis1, I A Rozovskaja, E Cragoe

  • 1Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.

FEBS Letters
|July 18, 1988
PubMed
Summary
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Cell attachment to solid surfaces triggers a rise in intracellular pH (pHi) via the Na+/H+ antiporter, essential for cell spreading and proliferation. This mechanism mirrors signaling by soluble growth factors.

Area of Science:

  • Cell Biology
  • Biochemistry
  • Biophysics

Background:

  • Activation of the Na+/H+ antiporter, increasing intracellular pH (pHi), is crucial for cell stimulation by soluble growth factors.
  • Solid substrates, like surfaces cells adhere to, can also act as growth factors, promoting cell proliferation.

Purpose of the Study:

  • To investigate the effect of cell attachment to a solid substrate on intracellular pH (pHi).
  • To determine the role of the Na+/H+ antiporter in pH changes upon cell adhesion.
  • To explore the relationship between substrate-induced pH changes and cell spreading.

Main Methods:

  • Measurement of intracellular pH (pHi) in mouse embryo fibroblasts.
  • Cell adhesion assays on solid substrates.
  • Investigation of Na+/H+ antiporter activity.

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Main Results:

  • Mouse embryo fibroblast attachment to solid substrate increased pHi by approximately 0.3 units.
  • The observed pH shift occurred post-adhesion and was necessary for cell spreading.
  • Evidence confirmed the involvement of the Na+/H+ antiporter in elevating pHi in adhered cells.

Conclusions:

  • Cellular response to physical growth factors (solid substrate) involves a similar signaling pathway to chemical growth factors (soluble ligands).
  • The Na+/H+ antiporter-mediated increase in pHi is a key event in cell adhesion and subsequent spreading.
  • This study highlights a conserved mechanism for growth factor signal transduction.