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Nano hydroxyapatite-blasted titanium surface affects pre-osteoblast morphology by modulating critical intracellular
Fábio Bezerra1, Marcel R Ferreira1, Giselle N Fontes2
1Department of Chemistry and Biochemistry, Bioscience Institute, State University of São Paulo-UNESP, P.O. Box: 510, 18618-970, Rubião Jr, campus Botucatu, São Paulo, Botucatu, Brazil.
Biotechnology and Bioengineering
|April 13, 2017
Summary
Nano hydroxyapatite-blasted titanium surfaces enhance pre-osteoblast adhesion and survival by modulating key intracellular pathways. This surface promotes cell spreading and anti-apoptotic effects, crucial for bone integration.
Area of Science:
- Biomaterials Science
- Cell Biology
- Tissue Engineering
Background:
- Intracellular signaling pathways influence cell-surface interactions.
- Titanium (Ti) surface topography affects biological responses.
- Nano hydroxyapatite (nHA) is a component of bone, suggesting potential biocompatibility.
Purpose of the Study:
- To investigate pre-osteoblast behavior on nano hydroxyapatite (nHA)-blasted titanium (Ti) surfaces.
- To explore critical intracellular pathways and morphological changes in response to Ti surface texturization.
- To evaluate the impact of surface physicochemical properties on osteoblast performance.
Main Methods:
- Surface characterization using atomic force microscopy (AFM) and water contact angle measurements.
- Seeding pre-osteoblast cells on machined (Mac), dual acid-etching (DAE), and nHA-blasted Ti surfaces.
- Analysis of cell morphology, apoptosis (Bax/Bcl2 ratio), adhesion (crystal violet assay), and signaling pathways (immunoblotting for FAK, Src, Ras-Erk).
- Assessment of osteoblast differentiation via alkaline phosphatase (ALP) activity and osteogenic transcription factors.
Main Results:
- nHA-blasted Ti surfaces exhibited distinct topography and wettability.
- Pre-osteoblasts showed enhanced spreading on nHA surfaces.
- nHA surfaces promoted a lower Bax/Bcl2 ratio, indicating anti-apoptotic effects.
- nHA surfaces increased pre-osteoblast adhesion by up-modulating FAK and Src activation.
- All Ti surfaces stimulated Ras-Erk signaling.
- All tested Ti surfaces promoted osteoblast differentiation.
Conclusions:
- Nano hydroxyapatite-blasted titanium surfaces promote crucial intracellular signaling networks for cell adaptation.
- The nHA surface enhances pre-osteoblast adhesion, survival, and differentiation.
- Surface topography and composition play a significant role in dictating cellular responses for bone regeneration applications.