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A Tumor Suppressor Gene Product, Platelet-Derived Growth Factor Receptor-Like Protein Controls Chondrocyte

Kazumi Kawata1, Satoshi Kubota1,2, Takanori Eguchi1

  • 1Department of Biochemistry and Molecular Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1, Shikata-cho, Kita-ku, Okayama-City, Okayama, 700-8525, Japan.

Journal of Cellular Biochemistry
|April 14, 2017
PubMed

Insights

Platelet-derived growth factor receptor-like (PDGFRL) gene promotes chondrocyte proliferation but inhibits breast cancer cell growth. PDGFRL plays distinct roles in different cell types, with a novel function in chondrocytes.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Gene Function

Background:

  • The platelet-derived growth factor receptor-like (PDGFRL) gene is traditionally considered a tumor suppressor.
  • The precise molecular functions of PDGFRL remain largely uncharacterized.

Purpose of the Study:

  • To elucidate the molecular function of PDGFRL, particularly within chondrocytes.
  • To investigate the differential roles of PDGFRL in various cell types.

Main Methods:

  • Gene expression analysis of PDGFRL mRNA in different cell lines and cartilage tissues.
  • Overexpression studies of PDGFRL in chondrocytic HCS-2/8 and breast cancer MDA-MB-231 cells.
  • Assessment of cell proliferation and chondrocyte differentiation marker gene expression.

Main Results:

  • PDGFRL mRNA expression was highest in chondrocytic HCS-2/8 cells.
  • PDGFRL overexpression promoted proliferation in HCS-2/8 cells but inhibited it in MDA-MB-231 cells.
  • PDGFRL overexpression decreased chondrocyte differentiation markers (SOX9, ACAN, COL2A1, COL10A1, ALP) in HCS-2/8 cells.

Conclusions:

  • PDGFRL exhibits cell-type-specific functions, promoting proliferation in chondrocytes while inhibiting it in breast cancer cells.
  • PDGFRL plays a novel and significant role in chondrocytes, distinct from its previously understood tumor suppressor function.
  • PDGFRL mRNA is expressed in normal chondrocytes, excluding hypertrophic chondrocytes, both in vitro and in vivo.
Keywords:
CELL PROLIFERATIONCHONDROCYTEDIFFERENTIATIONPDGFRL

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