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A multistage-dialysis microdevice for extraction of cryoprotectants
Lili Zou1,2, Shibo Li1,2, Yufeng Kang1,2
1Center for Biomedical Engineering, University of Science and Technology of China, Hefei, Anhui, 230027, China.
Biomedical Microdevices
|April 20, 2017
Summary
This study introduces a multistage-dialysis microdevice for efficient cryoprotectant removal from cell suspensions. The microdevice achieves high cell survival and recovery rates, crucial for lab-on-a-chip applications.
Area of Science:
- Biomedical Engineering
- Microfluidics
- Cell Biology
Background:
- Cryoprotective agents (CPAs) are essential for cell cryopreservation but must be removed before use.
- Efficient CPA extraction from small cell volumes is challenging for lab-on-a-chip applications.
Purpose of the Study:
- To develop and evaluate a multistage-dialysis microdevice (MDM) for CPA extraction.
- To optimize MDM performance by investigating key operational parameters.
Main Methods:
- A novel multistage-dialysis microdevice (MDM) was designed and fabricated.
- MDM functionality was validated using fluorescence solutions and glycerin-loaded swine erythrocytes.
- The effects of flow rate, CPA concentration, cell density, and membrane pore size on CPA clearance, cell survival, and recovery were systematically investigated.
Main Results:
- The MDM achieved approximately 60% glycerin clearance (CG), 90% cell survival (SC), and 70% cell recovery (RC) under optimized conditions.
- Higher flow rates improved SC and RC but reduced CG.
- Lower glycerin concentrations, lower cell densities, and larger membrane pore sizes generally increased CG but decreased SC and RC.
Conclusions:
- The developed MDM is effective for inline CPA extraction from cell suspensions.
- Understanding parameter effects allows for optimization of CPA removal for downstream cell applications.
- This microfluidic approach facilitates the integration of cryopreserved cells into lab-on-a-chip systems.