Related Experiment Videos
Characterization of apoB, E receptor function in the luteinized ovary
1Department of Medicine, Stanford University School of Medicine, Palo Alto, CA 94305.
Journal of Lipid Research
|July 1, 1988
Summary
Luteinized ovaries obtain much cholesterol from low-density lipoprotein (LDL) via surface extraction, not solely through apoB, E receptor pathways. This suggests a novel mechanism for cholesterol delivery to ovarian cells.
Area of Science:
- Reproductive Endocrinology
- Lipid Metabolism
- Cell Biology
Background:
- Luteinized ovarian cells may acquire cholesterol from low-density lipoprotein (LDL) without particle internalization.
- Lipoproteins are trapped in microvillar channels on luteal cell surfaces, suggesting a role in cholesterol transfer.
Purpose of the Study:
- To differentiate between classical receptor-mediated uptake and surface extraction for human LDL (hLDL) cholesterol transfer in perfused ovarian tissue.
- To investigate the role of apoB, E receptors in ovarian hLDL-cholesterol uptake during steroidogenesis.
Main Methods:
- Examined luteinized ovaries for apoB, E receptor presence.
- Utilized modified hLDL particles to characterize interactions with apoB, E receptors.
- Employed nonmetabolizable radiolabels to quantify cholesterol and protein uptake from hLDL.
Main Results:
- Luteinized ovaries possess apoB, E receptor protein.
- Modified hLDL, unable to bind apoB, E receptors, still delivered cholesterol for luteal cell uptake and steroidogenesis.
- hLDL interaction with luteinized ovarian tissue is atypical.
Conclusions:
- The apoB, E receptor pathway is not the primary route for LDL-cholesterol delivery to the luteinized ovary.
- Surface extraction mechanisms likely play a significant role in providing cholesterol for ovarian steroidogenesis.