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Related Concept Videos

Immunogold Electron Microscopy01:20

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Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
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The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
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In 1931, physicist Ernst Ruska—building on the idea that magnetic fields can direct an electron beam just as lenses can direct a beam of light in an optical microscope—developed the first prototype of the electron microscope. This development led to the development of the field of electron microscopy. In the transmission electron microscope (TEM), electrons are produced by a hot tungsten element and accelerated by a potential difference in an electron gun, which gives them up to 400...
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A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
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In situ TEM of Biological Assemblies in Liquid
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In situ TEM of Biological Assemblies in Liquid

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Perspectives on in situ electron microscopy.

Haimei Zheng1, Yimei Zhu2

  • 1Materials Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Department of Materials Science and Engineering, University of California, Berkeley, CA 94720, USA.

Ultramicroscopy
|April 25, 2017
PubMed
Summary
This summary is machine-generated.

In situ transmission electron microscopy (TEM) advances enable high-resolution observation of dynamic material processes. This technique is revolutionizing fields like materials science, chemistry, physics, and biology.

Keywords:
Aberration correctionColloidal nanocrystal growthFerroelectric vorticesFerromagnetic domains switchingIn situ TEMLiquid cell TEM

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Area of Science:

  • Materials Science
  • Chemistry
  • Physics
  • Biology

Background:

  • In situ transmission electron microscopy (TEM) offers high spatial and temporal resolution for observing dynamic material processes.
  • Recent advancements include liquid/gas environments, ultrafast microscopy, nanomechanics, and improved electron optics/detectors.

Purpose of the Study:

  • To highlight the development and applications of in situ TEM.
  • To showcase its potential across diverse scientific disciplines.

Main Methods:

  • Liquid environment electron microscopy for colloidal nanoparticle growth and electrochemical processes.
  • In situ studies of topological vortices in ferroelectric and ferromagnetic materials.

Main Results:

  • Demonstrated capability of in situ TEM in observing dynamic processes in liquids and solids.
  • Successful application in studying nanoparticle formation and material domain switching.

Conclusions:

  • In situ TEM is a powerful tool with expanding applications in materials science and beyond.
  • Future developments promise even greater insights into dynamic material behaviors.