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Published on: March 30, 2019
MiR-23b controls ALDH1A1 expression in cervical cancer stem cells
Weiwen Wang1,2, Yang Li1, Na Liu3
1Department of Gynecology and Obstetrics, First Affiliated Hospital, Xi'an Jiaotong University, 277 West Yanta Road, 710061, Xi'an, China.
Background:
Cancer stem cells has been widely investigated due to its essential role in cancer progression and drug resistance. Here, we try to find a new therapeutic target for cervical cancer stem cells.
Methods:
We detected ALDH1A1-associated miRNAs expression in our isolated tumorspheres and their corresponding parental cells. Sphere formation assay was also used to determine stemness after cells were manipulated with miR-23b plasmid or miR-23b inhibitor.
Results:
We found that miR-23b was under-expressed in cervical cancer stem cells to maintain high levels of ALDH1A1. Introduction of miR-23b into cervical cancer cells could alter stemness and cisplatin sensitivity.
Conclusions:
miR-23b plays key role in maintaining stemness of cervical cancer stem cells and can be developed as therapeutic target to better fight against cervical cancer.
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