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Ribosome Profiling

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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Updated: Mar 3, 2026

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

Published on: December 21, 2017

Ribosome profiling the cell cycle: lessons and challenges.

Rodolfo Aramayo¹, Michael Polymenis²

¹Department of Biology, Texas A&M University, College Station, TX, 77845, USA. raramayo@tamu.edu.

Current Genetics

|April 29, 2017

Summary

This summary is machine-generated.

Researchers studied gene expression control during the cell cycle by examining mRNA translation. This analysis revealed new biological insights into cell division regulation, though challenges remain for future studies.

Keywords:

Anota Babel Elutriation Translational efficiency

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Genome-wide Quantification of Translation in Budding Yeast by Ribosome Profiling

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Published on: December 21, 2017

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Area of Science:

Molecular Biology
Cell Biology
Genetics

Background:

Cell cycle studies typically focus on the abundance and activity of cellular components.
Control of gene expression during the cell cycle is crucial for proper cellular function.
Regulation at the translational level, specifically mRNA translation, is a key area of investigation.

Purpose of the Study:

To investigate the control of gene expression during the cell cycle at the level of translation.
To apply recent advancements in sequencing technologies to analyze translational regulation.
To identify transcripts under translational control across different cell cycle phases.

Main Methods:

Utilizing deep sequencing methodologies to quantify ribosome-bound mRNAs engaged in translation.
Applying ribosome profiling (or Ribo-seq) to synchronous cell populations.
Analyzing transcript levels across distinct phases of the cell cycle.

Main Results:

Identification of specific transcripts regulated at the translational level during the cell cycle.
New biological insights into the mechanisms of cell cycle control.
Demonstration of the utility and limitations of current ribosome profiling techniques.

Conclusions:

Translational control plays a significant role in cell cycle regulation.
Ribosome profiling is a powerful tool for studying gene expression dynamics.
Future research should address the limitations identified in current methodologies to advance understanding of cell cycle translational control.