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Updated: Mar 3, 2026
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
36.0K
CRISPR Editing Technology in Biological and Biomedical Investigation
Martyn K White1, Rafal Kaminski1, Won-Bin Young1
1Center for Neurovirology and Comprehensive NeuroAIDS Center, Department of Neuroscience, Lewis Katz School of Medicine at Temple University, 3500 N. Broad Street, Philadelphia, Pennsylvania, 19140.
Journal of Cellular Biochemistry
|May 2, 2017
Summary
The CRISPR gene editing system offers precise genetic manipulation and has evolved from a bacterial immune mechanism. This technology shows promise for research, gene therapy, and combating viral infections like HIV-1.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR (clustered regularly interspaced short palindromic repeats) originated as a prokaryotic immune system.
- It provides acquired immunity against foreign genetic elements like bacteriophages.
- CRISPR has been repurposed as a powerful genome editing tool.
Purpose of the Study:
- To describe the features of the CRISPR system.
- To highlight drawbacks and considerations for CRISPR applications.
- To discuss recent technical advancements and applications, including viral elimination.
Main Methods:
- Review of CRISPR system's mechanism and applications.
- Emphasis on strategies to mitigate off-target effects and improve delivery.
- Discussion of recent technical improvements and emerging uses.
Main Results:
- CRISPR enables specific, effective, and versatile genetic manipulation.
- The system is user-friendly, flexible, and amenable to technical improvements.
- Applications span research, gene therapy, and targeting human viruses.
Conclusions:
- CRISPR technology is a leading genome editing tool with broad applicability.
- Addressing off-target events and delivery efficiency are key challenges.
- CRISPR holds significant potential for therapeutic interventions, including antiviral strategies.
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