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Evidence of gene amplification in tunicamycin-resistant Chinese hamster ovary cells
J R Scocca1, K O Hartog, S S Krag
1Department of Biochemistry, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, Maryland.
Biochemical and Biophysical Research Communications
|November 15, 1988
Abstract:
Restriction digests of genomic DNA from tunicamycin-resistant Chinese hamster ovary cells, 3E11, were probed with the yeast transferase gene, ALG7. The data presented suggest moderate amplification of the N-acetylglucosaminyl-1-phosphate transferase gene occurred in these cells, consistent with the previously observed chromosomal translocations and increased enzymatic activity. This is the first example of gene amplification as a mechanism for aberrations in N-linked glycosylation.