Calculation of individual cleavage rates from partial digests in restriction endonuclease kinetics

A correction for results obtained by an analysis of DNA molecules partially cleaved with restriction endonuclease was suggested. The correction was proved on model data. Applications to (i) electron-microscopic analysis of singly cleaved molecules, (ii) partial digestion of a circular molecule followed by complete digestion with a second enzyme, (iii) systems with great cleavage differences, and (iv) partial cleavage of end-labelled molecules were discussed.