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Oncogenic function of TUSC3 in non-small cell lung cancer is associated with Hedgehog signalling pathway

Ye Gu1, Xiaojuan Pei2, Yansong Ren1

  • 1Department of Pathology, Nanfang Hospital & School of Basic Medicine, Southern Medical University, 1838 Guangzhou Avenue, Guangzhou 510515, PR China; Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, 1838 Guangzhou Avenue, Guangzhou 510515, PR China.

Biochimica Et Biophysica Acta. Molecular Basis of Disease
|May 11, 2017
PubMed

Insights

Tumour suppressor candidate 3 (TUSC3) is upregulated in non-small cell lung cancer (NSCLC), promoting tumour growth and metastasis. TUSC3 may drive NSCLC progression via the Hedgehog signalling pathway.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Non-small cell lung cancer (NSCLC) is a leading cause of cancer mortality.
  • Tumour suppressor candidate 3 (TUSC3) plays a role in cellular functions and is dysregulated in epithelial cancers.

Purpose of the Study:

  • To investigate the role of TUSC3 in NSCLC.
  • To explore the mechanism by which TUSC3 affects NSCLC progression.

Main Methods:

  • Analysis of TUSC3 expression in NSCLC tissues and cells.
  • In vitro and in vivo experiments to assess the functional impact of TUSC3.
  • Western blotting, co-immunoprecipitation, and immunofluorescence to study protein interactions and pathway involvement.

Main Results:

  • TUSC3 expression is upregulated in NSCLC and correlates with metastasis and survival.
  • TUSC3 overexpression enhances NSCLC cell proliferation, migration, invasion, and tumour growth.
  • TUSC3 interacts with GLI1, suggesting involvement in the Hedgehog signalling pathway.

Conclusions:

  • TUSC3 acts as an oncogene in NSCLC.
  • TUSC3 dysregulation contributes to NSCLC cell invasion and migration, potentially through the Hedgehog pathway.
Keywords:
Hh signalling pathwayNSCLCTUSC3

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