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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
Published on: May 3, 2018
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A bioactive peptide amidating enzyme is required for ciliogenesis.
Dhivya Kumar1, Daniela Strenkert2, Ramila S Patel-King1
1Department of Molecular Biology and Biophysics, University of Connecticut Health Center, Farmington, United States.
Elife
|May 18, 2017
Summary
Peptidylglycine α-amidating monooxygenase (PAM) is essential for cilia assembly across species. This enzyme
Area of Science:
- Cell Biology
- Molecular Biology
- Biochemistry
Background:
- Cilium biogenesis pathways are not fully understood.
- Peptidylglycine α-amidating monooxygenase (PAM) is crucial for bioactive peptide amidation.
- PAM has been identified in both Chlamydomonas and mammalian cilia.
Purpose of the Study:
- To investigate the role of PAM in cilia assembly.
- To elucidate the conserved function of PAM in ciliogenesis across different organisms.
Main Methods:
- Utilized Chlamydomonas knockdown models.
- Analyzed planarian gene expression.
- Examined primary cilia in mouse embryos.
- Assessed Golgi architecture and cilia assembly components.
Main Results:
- Chlamydomonas PAM knockdown disrupted cilia assembly beyond the transition zone and altered Golgi structure.
- Reduced PAM expression in planaria led to decreased motile ciliary density and cytosolic axonemes.
- Primary cilia in Pam mouse embryos exhibited abnormal architecture.
Conclusions:
- PAM is required for normal motile and primary cilia assembly in diverse species.
- PAM activity and post-Golgi trafficking defects contribute to ciliogenesis issues.
- A conserved link exists between PAM, amidation, and ciliary assembly.
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