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In vitro expression of the alpha and beta subunits of the Na,K-ATPase
M Gilmore-Hebert1, R W Mercer, J W Schneider
1Department of Internal Medicine, Yale University School of Medicine, New Haven CT.
Progress in Clinical and Biological Research
|January 1, 1988
Abstract:
Our analysis of the cloned alpha 3 protein strongly suggests that this c-DNA represents a bona fide Na+,K+ ATPase isoform. Its similarity to alpha + may have made its detection in tissues by gel migration or immunoreactivity difficult. Expression of an enzymatically active alpha 3 beta Na+K+ATPase either in a completely in vitro system or in a heterologous tissue culture system will clearly establish the biochemical properties of this isoform. Development of alpha 3 specific immunochemical probes will allow a proper assessment of its in vivo expression.