Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

10.0K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
10.0K
Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

8.7K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
8.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Building Towards Initiation, Moderation, De-Escalation and Cessation of Disease-Modifying Treatments for Multiple Sclerosis in Greece: An Expert Panel Consensus Meeting.

Brain sciences·2026
Same author

Indocyanine green near-infrared fluorescence angiography in free parascapular flaps: Distal flap perfusion assessment in a retrospective study of 112 cases.

JPRAS open·2026
Same author

Midazolam versus dexmedetomidine: breakthrough use variations-the DREAMS trial secondary analysis.

BMJ supportive & palliative care·2026
Same author

Decoding extremophiles: insights from bioinformatics, machine learning, and data-driven approaches.

Briefings in bioinformatics·2026
Same author

Liquid biopsies for the diagnosis of early-stage gastric cancer: A 5-year systematic review.

The journal of liquid biopsy·2026
Same author

[Role and Techniques of Peripheral Nerve Surgery in Orthoplastic Surgery].

Handchirurgie, Mikrochirurgie, plastische Chirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Handchirurgie : Organ der Deutschsprachigen Arbeitsgemeinschaft fur Mikrochirurgie der Peripheren Nerven und Gefasse : Organ der V...·2026
Same journal

Correction to 'scSuperAnnotator: A platform for benchmarking comparison and visualizing automated cellular annotation methods for scRNA-seq data'.

Nucleic acids research·2026
Same journal

Correction to 'Differentiable partition function calculation for RNA'.

Nucleic acids research·2026
Same journal

Deployment of non-canonical splicing in tunicate genomes is mediated by divergent U2AF function and changing m6A modification in U1 and U6 snRNA.

Nucleic acids research·2026
Same journal

Bacillus subtilis DnaB forms multiple protein-protein interactions essential for DNA replication initiation.

Nucleic acids research·2026
Same journal

Multiple forms of protein-protein and DNA binding are exhibited by BrxC from the BREX phage restriction system.

Nucleic acids research·2026
Same journal

Biosynthesis of glycosylated 5-hydroxycytosine in the DNA of diverse viruses.

Nucleic acids research·2026
See all related articles

Related Experiment Video

Updated: Mar 2, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

1.1K

ProteoSign: an end-user online differential proteomics statistical analysis platform.

Georgios Efstathiou1,2, Andreas N Antonakis1, Georgios A Pavlopoulos1,3

  • 1Medical School, Division of Basic Sciences, University of Crete, Heraklion 71003, Greece.

Nucleic Acids Research
|May 19, 2017
PubMed
Summary
This summary is machine-generated.

Proteomics differential expression analysis is simplified with ProteoSign, a new web application. This tool makes complex mass spectrometry data analysis user-friendly for researchers without extensive programming skills.

More Related Videos

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

1.4K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.4K

Related Experiment Videos

Last Updated: Mar 2, 2026

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools
07:01

Navigating the Mass Spectrometry-Based Proteomic Data Using Free Computational Tools

Published on: August 19, 2025

1.1K
A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions
09:00

A Streamlined Approach for Mass Spectrometry-Based Proteomics Using Selected Tissue Regions

Published on: April 18, 2025

1.4K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

7.4K

Area of Science:

  • Proteomics
  • Bioinformatics
  • Computational Biology

Background:

  • Proteome dynamics are key to understanding cellular responses and homeostasis.
  • Mass spectrometry (MS) is a powerful tool for protein identification and characterization.
  • Analyzing large proteomics datasets from MS experiments presents significant data management and processing challenges.

Purpose of the Study:

  • To introduce ProteoSign, a freely available web application for user-friendly proteomics differential expression/abundance analysis.
  • To overcome the technical barriers associated with existing proteomics data analysis tools.
  • To provide a resource-efficient solution for proteomics laboratories and core facilities.

Main Methods:

  • Development of a web-based application, ProteoSign.
  • Implementation of a user-friendly and self-explanatory interface for data analysis.
  • Focus on post-quantification statistical analysis of proteomics data.

Main Results:

  • ProteoSign offers an accessible platform for statistical analysis of quantitative proteomics datasets.
  • The web application simplifies complex data processing, avoiding stringent software installations.
  • Enables efficient analysis for researchers lacking advanced programming expertise.

Conclusions:

  • ProteoSign addresses the need for user-friendly proteomics data analysis tools.
  • The application facilitates the statistical analysis of differential expression in proteomics studies.
  • It empowers laboratories to analyze complex MS data more efficiently and accessibly.