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Bioengineering bacterial outer membrane vesicles as vaccine platform.

Matthias J H Gerritzen1, Dirk E Martens2, René H Wijffels3

  • 1Institute for Translational Vaccinology (Intravacc), Process Development Bacterial Vaccines, P.O. Box 450, 3720 AL Bilthoven, The Netherlands; Wageningen University, Bioprocess Engineering, P.O. Box 16, 6700 AA Wageningen, The Netherlands.

Biotechnology Advances
|May 20, 2017
PubMed
Summary
This summary is machine-generated.

Outer membrane vesicles (OMVs) offer a versatile vaccine platform. Engineering these bacterial nanoparticles with foreign antigens enhances immunogenicity and allows for targeted immune responses against various diseases.

Keywords:
BioengineeringExtracellular vesiclesNanobiotechnologyOuter membrane vesiclesVaccine platform

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Area of Science:

  • Microbiology
  • Immunology
  • Nanotechnology

Background:

  • Outer membrane vesicles (OMVs) are naturally occurring nanoparticles from Gram-negative bacteria.
  • OMVs are highly immunogenic and have been utilized in vaccines against bacterial meningitis and sepsis.
  • The composition of OMVs can be genetically modified for therapeutic applications.

Purpose of the Study:

  • To review the potential of engineered OMVs as a vaccine platform.
  • To discuss design considerations for OMV-based vaccines displaying heterologous antigens.
  • To explore antigen location, adjuvant properties, physiochemical characteristics, and immune responses.

Main Methods:

  • Review of existing literature on OMV vaccine platforms.
  • Analysis of strategies for engineering heterologous antigens onto OMVs.
  • Discussion of factors influencing vaccine efficacy and immunogenicity.

Main Results:

  • Engineered OMVs can display heterologous antigens while maintaining native conformation.
  • This approach allows for targeted immune responses and a streamlined vaccine production process.
  • Several promising OMV-based vaccine concepts have been developed.

Conclusions:

  • Engineered OMVs represent a promising and adaptable vaccine platform.
  • Careful consideration of design parameters is crucial for optimizing OMV vaccine efficacy.
  • Further research into OMV vaccine design can lead to novel therapeutics.