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Engineering Eschericha coli for Enhanced Tyrosol Production.
Yuxiang Xue1,2, Xianzhong Chen1,2, Cui Yang1,2
1Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education, Jiangnan University , Wuxi 214122, China.
Journal of Agricultural and Food Chemistry
|May 23, 2017
Summary
Microbial biosynthesis of tyrosol, a beneficial phenolic compound, was achieved in engineered Escherichia coli. Deleting specific genes and optimizing fermentation conditions significantly improved tyrosol production from glucose and tyrosine.
Area of Science:
- Biotechnology
- Metabolic Engineering
- Microbial Production
Background:
- Tyrosol, a phenolic compound in olive oil and wine, offers health benefits.
- Plant extraction of tyrosol is challenging, motivating microbial biosynthesis.
- Microbial production offers a sustainable alternative for tyrosol generation.
Purpose of the Study:
- To engineer Escherichia coli for efficient tyrosol production.
- To investigate the impact of gene deletions on tyrosol yield.
- To optimize fermentation conditions for enhanced tyrosol biosynthesis.
Main Methods:
- Introduction of phenylpyruvate decarboxylase (ARO10) and aromatic amino acid aminotransferase (ARO8) genes into E. coli.
- Deletion of prephenate dehydratase (pheA) and phenylacetaldehyde dehydrogenase (feaB) genes.
- Optimization of fermentation conditions and substrate utilization (glucose, tyrosine).
Main Results:
- Engineered E. coli strains successfully produced tyrosol.
- Deletion of pheA and feaB genes enhanced tyrosol production.
- A strain overexpressing ARO10 produced 4.15 mM tyrosol from 1% glucose in 48h.
- Co-overexpression of ARO8 and ARO10 yielded 8.71 mM tyrosol from 10 mM tyrosine.
Conclusions:
- Microbial biosynthesis of tyrosol is feasible using engineered E. coli.
- Metabolic engineering strategies can significantly improve tyrosol yield.
- This study demonstrates the potential for industrial-scale microbial tyrosol production.
