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Polymeric 3D Printed Functional Microcantilevers for Biosensing Applications.

Stefano Stassi1, Erika Fantino1, Roberta Calmo1

  • 1Department of Applied Science and Technology, Politecnico di Torino , Corso Duca degli Abruzzi 24, Torino 10129, Italy.

ACS Applied Materials & Interfaces
|May 23, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed novel 3D printed polymeric biosensors with built-in functionalities. These mass-sensitive microcantilever biosensors enable efficient biomolecule immobilization for applications like immunoassays.

Keywords:
3D printingDLPbiosensorsmechanical resonatorsmicrocantilever

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Area of Science:

  • Materials Science
  • Biotechnology
  • Microfabrication

Background:

  • Traditional biosensor fabrication can be complex and costly.
  • Developing biosensors with intrinsic functionalities is a key research area.
  • 3D printing offers potential for customized and integrated microdevice fabrication.

Purpose of the Study:

  • To demonstrate the first production of mass-sensitive polymeric biosensors using 3D printing.
  • To showcase the integration of intrinsic functionalities within 3D printed biosensors.
  • To validate the use of these biosensors in a standard immunoassay.

Main Methods:

  • Utilizing 3D printing technology for microcantilever fabrication.
  • Incorporating acrylic acid as a functional comonomer to introduce reactive groups.
  • Covalently immobilizing biomolecules onto the 3D printed polymer surface.
  • Implementing the biosensors in a standard immunoassay protocol.

Main Results:

  • Successfully produced mass-sensitive polymeric biosensors via a one-step 3D printing process.
  • Demonstrated controlled functionalization of the polymer for biomolecule immobilization.
  • Validated the performance of 3D printed microcantilever biosensors in an immunoassay.
  • Showcased the combination of 3D microfabrication, material characterization, and biosensor development.

Conclusions:

  • 3D printing enables the creation of engineered polymeric microcantilevers with intrinsic functionalities.
  • Tuning resin composition allows control over the functionalities of 3D printed devices.
  • This approach opens avenues for a new class of mass-sensing microelectromechanical system devices.