Related Experiment Videos
Affinity purification of the mammalian beta-2-adrenergic receptor
J L Tedesco1, K M Pearsall, M Thompson
1Department of Chemistry, University of Toronto, Mississauga, Canada.
Journal of Biochemical and Biophysical Methods
|November 1, 1988
Abstract:
The efficiency of covalently linking alprenolol to Sepharose via a 14-atom spacer prepared from 1,4-butanediol diglycidyl ether has been increased. This in turn has aided in increasing the specific yield of beta-2-adrenergic receptor by a factor of 3 over earlier results. Further development of extraction and solubilization protocols are also described. The adsorption of the affinity-purified receptor to stainless steel was measured, and is cited as a potential problem in further purification by high-pressure liquid chromatography.