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Fluorescent nanocellulosic hydrogels based on graphene quantum dots for sensing laccase
Celia Ruiz-Palomero1, Sandra Benítez-Martínez1, M Laura Soriano1
1Department of Analytical Chemistry, Institute of Fine Chemistry and Nanochemistry, Marie Curie Building, Campus de Rabanales, University of Córdoba, E-14071 Córdoba, Spain.
Analytica Chimica Acta
|May 25, 2017
Summary
A new fluorescent sensor using graphene quantum dots in nanocellulose hydrogels offers sensitive and selective detection of laccase enzyme. This eco-friendly platform provides a cost-efficient method for enzyme analysis and stabilization.
Area of Science:
- Biochemistry
- Materials Science
- Analytical Chemistry
Background:
- Laccase enzyme detection typically relies on catalytic activity, which is sensitive to environmental conditions.
- Existing methods may lack selectivity and stability, limiting their practical application.
- Graphene quantum dots (GQDs) offer unique fluorescent properties but can suffer from self-quenching.
Purpose of the Study:
- To develop a novel, low-cost, and sensitive fluorimetric platform for laccase enzyme detection.
- To utilize nanocellulosic hydrogels to enhance the performance of graphene quantum dots (GQDs).
- To demonstrate the selective detection of laccase in complex matrices like shampoos.
Main Methods:
- Fabrication of a fluorimetric sensor by immersing sulfur, nitrogen-codoped graphene quantum dots (GQDs) into nanocellulosic hydrogels.
- Utilizing the fluorescence quenching response of GQDs upon interaction with laccase.
- Investigating noncovalent interactions and energy transfer mechanisms for signal transduction.
- Assessing sensor performance in complex matrices, including shampoos.
Main Results:
- The nanocellulosic hydrogel matrix successfully improved GQD fluorescence, reduced self-quenching, and enhanced sensitivity.
- The sensor exhibited selective fluorescence quenching towards laccase via energy transfer without peak shifts.
- A low detection limit of 0.048 U mL⁻¹ was achieved for laccase detection.
- High recovery rates (86.2-94.1%) were obtained in complex shampoo samples.
Conclusions:
- The developed sensor is simple, eco-friendly, and cost-efficient, leveraging the properties of nanocellulose and GQDs.
- The platform enables sensitive and selective detection and stabilization of laccase.
- This strategy offers added value for enzyme storage, recycling, and analysis in real-world samples.
