Related Experiment Video
Updated: Mar 1, 2026
Author Spotlight: Comparing Alveolar and Long Bone Remodeling to Explore OTM Model Potential
Published on: July 21, 2023
Gene expression profile altered by orthodontic tooth movement during healing of surgical alveolar defect
Eun-Kyung Choi1, Jae-Hyung Lee2, Seung-Hak Baek3
1Graduate School, Kyung Hee University, Seoul, Korea.
Introduction:
We explored the gene expression profile altered by orthodontic tooth movement (OTM) during the healing of surgical alveolar defects in beagles.
Methods:
An OTM-related healing model was established where a maxillary second premolar was protracted into the critical-sized defect for 6 weeks (group DT6). As controls, natural healing models without OTM were set at 2 weeks (group D2) and at 6 weeks (group D6) after surgery. Total RNAs were extracted from dissected tissue blocks containing the regenerated defects and additionally from sound alveolar bone as a baseline (group C). mRNA profiling was performed using microarray analysis.
Results:
Functional annotations of gene clusters based on differentially expressed genes among groups indicated that the gene expression profile of group DT6 had a stronger similarity to that of group D2 than to group D6. The genes participating in high woven-bone fraction in group DT6 could be identified as TNFSF11, MMP13, SPP1, and DMP1, which were verified by quantitative real-time polymerase chain reactions.
Conclusions:
We investigated at the gene level that OTM can affect the healing state of surgical defects serving as favorable matrices for OTM with defect regeneration. It would be a basis on selecting putative genes to be therapeutically applied for tissue-friendly accelerated orthodontics in the future.
