Related Experiment Video
Updated: Mar 1, 2026
08:09
High-throughput Titration of Luciferase-expressing Recombinant Viruses
Published on: September 19, 2014
13.4K
A Semi-automated High-Throughput Microtitration Assay for Filoviruses
Claire Marie Filone1, David Miller1, Victoria Wahl-Jensen2
1Department of Homeland Security, National Biological Threat Characterization Center, National Biodefense Analysis & Countermeasures Center, Science & Technology Directorate, Fort Detrick, MD, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 3, 2017
Summary
Automated liquid handling and semi-automated readout significantly enhance the throughput of the 50% tissue culture infectious dose (TCID50) assay for filovirus infectivity measurement in BSL-4 environments.
Area of Science:
- Virology
- Biosafety Level 4 Research
- Assay Development
Background:
- The 50% tissue culture infectious dose (TCID50) assay is a gold standard for measuring filovirus infectivity.
- High-throughput filovirus infectivity assays are crucial for BSL-4 research.
Purpose of the Study:
- To increase the throughput of virology microtitration assays at BSL-4.
- To optimize the TCID50 assay for improved efficiency and data quality.
Main Methods:
- Implementation of automated liquid handling for cell plating and virus dilution.
- Semi-automated assay endpoint readout using a luminescent-based cell viability assay.
- Utilization of an automated plate reader for endpoint determination.
Main Results:
- Improved workflow efficiency in BSL-4 microtitration assays.
- Reduced operator burden and assay time.
- Decreased assay variability and increased data return.
Conclusions:
- Automated liquid handling and semi-automated readout enhance TCID50 assay throughput and efficiency.
- The optimized workflow provides more reliable and reproducible filovirus infectivity data.
- This approach is valuable for high-throughput virology in BSL-4 settings.
