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Stretching Short Sequences of DNA with Constant Force Axial Optical Tweezers
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Dynamic DNA Origami Device for Measuring Compressive Depletion Forces.

Michael W Hudoba1, Yi Luo, Angelita Zacharias

  • 1Department of Systems Engineering, Otterbein University , Westerville, Ohio 43081, United States.

ACS Nano
|June 6, 2017
PubMed
Summary

Researchers engineered DNA nanodevices with inherent structural dynamics. These nanodevices can sense and respond to their environment, enabling sensitive molecular-scale measurements.

Keywords:
DNA nanotechnologyDNA origamiconformational dynamicsdepletion forcesmolecular crowdingsingle-molecule biophysics

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Area of Science:

  • Nanotechnology
  • Molecular Engineering
  • Biophysics

Background:

  • Biomolecules exhibit responsive functions driven by conformational changes due to environmental interactions.
  • Self-assembling nanodevices with programmed dynamics are crucial for advanced applications.

Purpose of the Study:

  • To engineer DNA nanodevices with inherent nanoscale structural dynamics (nanodynamics).
  • To create devices capable of sensing and responding to the local environment with high sensitivity.
  • To establish design principles for regulating nanodynamic behavior.

Main Methods:

  • Engineering DNA devices with two stable conformational states and a transition state.
  • Utilizing single-molecule Förster resonance energy transfer (smFRET) measurements.
  • Investigating sensitivity to sub-piconewton depletion forces under molecular crowding.

Main Results:

  • Demonstrated spontaneous transitions between stable states in DNA nanodevices.
  • Showcased sensitivity of nanodynamics to sub-piconewton depletion forces.
  • Achieved measurement resolution of approximately 100 femtonewtons (fN) for depletion forces.

Conclusions:

  • Engineered nanodynamic DNA devices can mimic biomolecular responsiveness.
  • These devices offer a platform for molecular-scale sensing with high sensitivity.
  • The approach enables the development of responsive nanomachines for diverse applications.