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CAPRRESI: Chimera Assembly by Plasmid Recovery and Restriction Enzyme Site Insertion
Published on: June 25, 2017
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Draft Genome Sequences of Enterobacter cloacae Strains CAPREx E7 and CAPREx E2-2.
Rita E Monson1, Joseph Honger1, Alison Rawlinson1
1Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom.
Genome Announcements
|June 10, 2017
Summary
Two Enterobacter cloacae strains from Ghanaian yams in London were sequenced. Both strains are similar and carry genes for beta-lactamase enzymes, including AmpC.
Area of Science:
- Microbiology
- Genomics
- Antimicrobial Resistance
Background:
- Enterobacter cloacae is an opportunistic pathogen.
- Antimicrobial resistance is a growing global health concern.
- Food products can act as vehicles for pathogen transmission.
Purpose of the Study:
- To characterize two Enterobacter cloacae strains isolated from Ghanaian yams.
- To analyze the genomic features of these strains, focusing on antimicrobial resistance genes.
Main Methods:
- Isolation and identification of Enterobacter cloacae strains.
- Draft genome sequencing of the isolates.
- Bioinformatic analysis of genomic data, including G+C content and gene identification.
Main Results:
- Two similar Enterobacter cloacae strains, CAPREx E7 and CAPREx E2-2, were identified.
- The draft genomes were approximately 5 million base pairs with similar G+C content.
- Both strains possessed genes encoding three distinct beta-lactamases, including the AmpC type.
Conclusions:
- The genomic data provides insights into the genetic makeup of Enterobacter cloacae found in imported food.
- The presence of beta-lactamase genes highlights the potential for antimicrobial resistance in these foodborne bacteria.
- Further surveillance is warranted to understand the prevalence and implications of such strains in the food chain.
