Anesthetic-dependent changes in the chain-melting phase transition of DPPG liposomes studied using near-infrared
Marta Kuć1, Katarzyna Cieślik-Boczula1, Maria Rospenk1
1Faculty of Chemistry, University of Wroclaw, Joliot- Curie 14, 50-383 Wroclaw, Poland.
Abstract:
The effect of inhalation anesthetics (enflurane, isoflurane, sevoflurane or halothane) on the lipid chain-melting phase transition of negatively charged phospholipid membranes was studied using near-infrared (NIR) spectroscopy supported by Principal Component Analysis (PCA). NIR spectra of anesthetics-mixed dipalmitoylphosphatidylglycerol (DPPG) membranes were recorded in a range of the first overtone of the symmetric and antisymmetric stretching vibrations of CH2 groups of lipid aliphatic chains as a function of increasing temperature. Anesthetic-dependent changes in the trans to gauche conformers ratio of CH2 groups in the hydrocarbon lipid chains were characterized in detail and compared with the zwitterionic lipid membranes, which were built of dipalmitoylphosphatidylcholine (DPPC) molecules.
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