[Enhanced ILC2 activity contributes to metabolic syndrome multicomponent aggregation state in patients with type 2...

Objective To study the relationship between the activity of group II innate lymphoid cells (ILC2) in maintenance hemodialysis patients with type 2 diabetic nephropathy (DN) and metabolic syndrome (MS) multicomponent aggregation state. Methods The study enrolled 30 DN patients of stable condition treated with maintenance hemodialysis over 3 months, 30 newly diagnosed untreated type 2 diabetes patients and 30 healthy volunteers as controls. Real-time quantitative PCR (qRT-PCR) was used to detect the mRNA expression levels of retinoic acid receptor-related orphan receptor α (RORα), interleukin 17 receptor B (IL-17RB), IL-33 receptor T1/ST2, IL-5 and IL-13 in peripheral blood mononuclear cells (PBMCs). The percentage of ILC2 cells in PBMCs was determined by flow cytometry. Levels of plasma IL-5 and IL-13 were tested by ELISA. The prevalence rate and number of MS in the three groups were examined and the relationship between the components and the ILC2-related factors was analyzed. Results Patients diagnosed with type 2 diabetes or DN had higher waist circumference (WC), fasting blood glucose (FBG), systolic blood pressure (SBP), diastolic blood pressure (DBP), triglyceride (TG) and the number of MS components as compared with healthy controls. The prevalence rates of MS in type 2 diabetes group andDN group were 46.67% and 86.67%, respectively. The proportion of ILC2 increased and both ILC2-related transcription factor RORα and T1/ST2 mRNA expression levels ascended in DN patients as compared with type 2 diabetes patients and healthy controls. The correlation analysis showed that ILC2 receptor T1/ST2, IL-5, IL-13 mRNA levels were positively related to ILC2 transcription factor RORα mRNA levels in the DN group. IL-13 mRNA level in PBMCs was positively related to the levels of SBP, plasma total cholesterol (TC)and low density lipoprotein cholesterol (LDL-C) , and membrane receptor T1/ST2 mRNA level was positively related to the level of LDL-C. Conclusion MS multicomponent aggregation in maintenance hemodialysis patients with type 2 DN group was more obvious than that in type 2 diabetes mellitus group and healthy control group, and strengthened ILC2 activity might contribute to the formation of the state by influencing blood pressure and lipid metabolism.