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Restriction cleavage maps of coliphages 186 and P2
Molecular & General Genetics : MGG
|March 9, 1979
Summary
Restriction enzymes were used to map DNA cleavage sites in coliphages P2 and 186. This detailed analysis provides a foundational understanding of these related bacteriophages.
Area of Science:
- Molecular Biology
- Virology
- Genetics
Background:
- Coliphages P2 and 186 are related bacteriophages with distinct biological properties.
- Understanding their genome organization is crucial for molecular and genetic studies.
Purpose of the Study:
- To precisely map the cleavage sites of seven restriction enzymes within the genomes of coliphages P2 and 186.
- To generate a detailed restriction enzyme map for comparative genomic analysis.
Main Methods:
- DNA from coliphages P2 and 186 was digested with restriction enzymes BamHI, Bg/II, EcoRI, HindIII, PstI, XbaI, and XhoI.
- Cleavage sites were mapped using three distinct approaches: analysis of cohesive termini and hybrids, double digests, and partial digests with Southern blotting.
Main Results:
- The number of restriction sites for each enzyme in both P2 and 186 coliphages were determined.
- Most restriction sites were mapped, with the exception of PstI sites in the 186 coliphage control region.
Conclusions:
- A comprehensive restriction enzyme map was generated for coliphages P2 and 186.
- This map serves as a valuable resource for future genetic manipulation and comparative studies of these bacteriophages.