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Cytotoxic withanolides from Physalis angulata.

Caiyun Gao1, Ruijun Li1, Miaomiao Zhou1

  • 1a State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry , China Pharmaceutical University , Nanjing , People's Republic of China.

Natural Product Research
|June 16, 2017
PubMed
Summary
This summary is machine-generated.

A novel withanolide, physagulide P, was isolated from Physalis angulata. This compound demonstrated significant cytotoxic activity against human osteosarcoma, hepatoma, and breast cancer cell lines.

Keywords:
Cytotoxic activityPhysalis angulata LWithanolides

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Area of Science:

  • Natural Product Chemistry
  • Pharmacology
  • Medicinal Chemistry

Background:

  • Physalis angulata L. is a plant species known for its diverse phytochemical constituents.
  • Withanolides are a class of steroidal lactones with a wide range of biological activities.
  • Exploring novel compounds from natural sources is crucial for drug discovery.

Purpose of the Study:

  • To isolate and characterize new withanolides from the aerial parts of Physalis angulata L.
  • To evaluate the cytotoxic potential of the isolated compounds against various human cancer cell lines.

Main Methods:

  • Isolation of compounds using chromatographic techniques.
  • Structure elucidation of the new compound (physagulide P) employing 1D, 2D NMR, and HRESIMS.
  • Cytotoxicity assays using MG-63 (osteosarcoma), HepG-2 (hepatoma), and MDA-MB-231 (breast cancer) cell lines.

Main Results:

  • A new withanolide, physagulide P (1), was successfully isolated along with five known withanolides (2-6).
  • The structure of physagulide P was confirmed through comprehensive spectroscopic analysis.
  • Physagulide P exhibited significant cytotoxicities with IC50 values of 3.50 μM against MG-63, 4.22 μM against HepG-2, and 15.74 μM against MDA-MB-231 cells.

Conclusions:

  • Physalis angulata L. is a valuable source of bioactive withanolides.
  • Physagulide P is a promising new compound with potent cytotoxic effects on multiple human cancer cell lines.
  • Further investigation into physagulide P could lead to the development of novel anticancer therapeutics.