Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Safety and Immunogenicity of the Live-Attenuated Quadrivalent Dengue Vaccine V181 Compared With Butantan-DV Among Healthy Adults in Brazil: A Randomized, Double-Blind, Phase 2 Trial.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2026
Same author

High Intensity Interval Training in Aged Female Mice Preserves Physical, Cognitive, and Cardiovascular Function.

bioRxiv : the preprint server for biology·2026
Same author

Dengue Incidence, Seroprevalence, and Expansion Factors from Active Surveillance, Brazil, 2016-2021.

Emerging infectious diseases·2026
Same author

Valorization of Canola Leaves (Brassica napus L.) Through Sourdough Breadmaking: A Preliminary Study on Dough Rheology and Bread Quality.

Plant foods for human nutrition (Dordrecht, Netherlands)·2026
Same author

Scientific evidence does not support oyster farming as a marine carbon dioxide removal strategy for climate mitigation.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

The kinetics and transmission-blocking dynamics of <i>P. falciparum</i> sexual-stage antibody responses in a six-year cohort of Ugandan children and adults.

Research square·2026

Related Experiment Video

Updated: Feb 28, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

12.5K

Highly Multiplex Real-Time PCR-Based Screening for Blood-Borne Pathogens on an OpenArray Platform.

Elena Grigorenko1, Carolyn Fisher2, Sunali Patel3

  • 1Department of Research and Development, Diatherix Laboratories, LLC, Huntsville, Alabama.

The Journal of Molecular Diagnostics : JMD
|June 18, 2017
PubMed
Summary
This summary is machine-generated.

A new molecular diagnostic tool accurately detects multiple blood-borne pathogens in blood products. This highly multiplexed assay offers flexibility for emerging infectious agents, enhancing blood safety.

More Related Videos

Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.8K
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

13.3K

Related Experiment Videos

Last Updated: Feb 28, 2026

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR
11:00

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Published on: November 28, 2016

12.5K
Visual Detection of Multiple Nucleic Acids in a Capillary Array
08:56

Visual Detection of Multiple Nucleic Acids in a Capillary Array

Published on: November 15, 2017

7.8K
Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray
07:35

Demonstrating a Multi-drug Resistant Mycobacterium tuberculosis Amplification Microarray

Published on: April 25, 2014

13.3K

Area of Science:

  • Molecular diagnostics
  • Blood bank industry
  • Pathogen detection

Background:

  • Molecular diagnostics are vital in blood banking.
  • A need exists for flexible, simultaneous pathogen detection in blood products.
  • Emerging pathogens require adaptable testing solutions.

Purpose of the Study:

  • To develop a highly multiplexed blood-borne pathogen panel (BBPP) for simultaneous detection of multiple pathogens.
  • To validate the BBPP on the OpenArray platform for human plasma and blood samples.
  • To assess the device's potential as a next-generation blood product testing solution.

Main Methods:

  • Development of a dual-label probe chemistry (TaqMan assays) BBPP.
  • Utilizing the OpenArray platform for high-throughput PCR.
  • Testing with purified nucleic acids, pathogen-spiked samples, and clinical blood donor samples.

Main Results:

  • The BBPP accurately detected 17 viral and 13 bacterial/protozoan pathogens.
  • Analytical validation showed high specificity with no false positives in coded samples.
  • Clinical sample testing correctly identified 95% of virus-positive samples.

Conclusions:

  • The OpenArray PCR platform can be adapted for high-throughput, multiplexed pathogen detection.
  • The developed BBPP demonstrates potential for enhanced blood product safety.
  • This technology offers flexibility for incorporating tests for newly emerging infectious agents.