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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.5K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Related Experiment Video

Updated: Feb 28, 2026

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
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Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

Published on: November 6, 2021

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Time-resolved cryo-electron microscopy: Recent progress.

Joachim Frank1

  • 1Howard Hughes Medical Institute, Department of Biochemistry and Molecular Biophysics, Columbia University, 650 W. 168th Street, New York, NY 10032, United States; Department of Biological Sciences, Columbia University, United States.

Journal of Structural Biology
|June 20, 2017
PubMed
Summary
This summary is machine-generated.

Time-resolved cryo-electron microscopy (cryo-EM) captures molecular reactions over time. This technique merges structural insights with kinetic analysis for biological studies.

Keywords:
Cryo-EMMicrofluidicsRRFRibosome recyclingShort-lived states

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Area of Science:

  • Biophysics
  • Structural Biology
  • Biochemistry

Background:

  • Single-particle cryo-electron microscopy (cryo-EM) excels at determining molecular structures.
  • Understanding dynamic molecular processes requires methods that capture transient states.

Purpose of the Study:

  • To outline recent advancements in time-resolved cryo-EM.
  • To highlight the initial biological applications of this technique.

Main Methods:

  • Time-resolved cryo-electron microscopy (cryo-EM) integrates rapid mixing or perturbation with cryo-EM data collection.
  • Analysis of time-series data to visualize structural changes during reactions.

Main Results:

  • Demonstration of cryo-EM's capability to capture dynamic molecular events.
  • Successful application in studying in vitro molecular reactions.

Conclusions:

  • Time-resolved cryo-EM is a powerful tool for investigating molecular mechanisms.
  • This methodology opens new avenues for studying biological processes at a molecular level.