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Updated: Feb 28, 2026
Culture of Bladder Cancer Organoids as Precision Medicine Tools
Published on: December 28, 2021
[THE MAIN STAGES OF THE PREPARATION OF VIABLE TUMOR CELLS OF BLADDER CANCER: FROM ISOLATION OF THE TUMOR MATERIAL
T Slavyanskaya1, S -Salnikova1
11 People's Friendship University of Russia (RUDN University); 2 Institute of Immunophysiology; 3 FSBI Clinical Hospital №1 of the Presidential Affairs Management of the Russian Federation, Moscow, Russian Federation.
Abstract:
The development of personalized autologous dendritic cell anti-tumor vaccines (PDPW) against bladder cancer (BC) is a relevant issue that covers many aspects required for its standardization. The article presents personal experimental studies related to development of optimal conditions of transportation of biological material; temperature and temporary modes for the storage of the samples; the materials about the optimal method of disaggregation of the biomaterial; there has been shown a comparative analysis of different methods of tumor disaggregation; the selection of nutritious growth mediums and growth factors for urothelial carcinoma cells (UCC), the conditions of cryopreservation of tumor cells for maximum UCC viability, potentially suitable for creating PDPW against BC.
