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Interaction between Pasteurella multocida B:2 and its derivatives with bovine aortic endothelial cell (BAEC)
Nuriqmaliza M Kamal1, M Zamri-Saad2, Mas Jaffri Masarudin1
1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
BMC Veterinary Research
|June 21, 2017
Summary
Pasteurella multocida B:2 GDH7 shows enhanced adherence and invasion capabilities in bovine cells, suggesting its potential as a live attenuated vaccine delivery vehicle for future multivalent DNA vaccines against haemorrhagic septicaemia.
Area of Science:
- Veterinary Microbiology
- Vaccinology
- Bacterial Pathogenesis
Background:
- Pasteurella multocida B:2 is a significant cause of bovine haemorrhagic septicaemia (HS), a fatal disease in cattle and buffaloes.
- An attenuated strain, P. multocida B:2 GDH7, derived from P. multocida B:2, has previously demonstrated efficacy as a live attenuated vaccine for HS.
- Current live attenuated vaccine candidates for HS are limited, with P. multocida B:2 GDH7 and P. multocida B:2 JRMT12 being the primary options.
Purpose of the Study:
- To evaluate the potential of the P. multocida B:2 GDH7 strain as a delivery vehicle for DNA vaccines.
- To explore future multivalent vaccine applications using P. multocida B:2 GDH7.
- To investigate the in vitro interaction of P. multocida B:2 GDH7 with bovine cells.
Main Methods:
- Assessing adherence, invasion, and intracellular survival of P. multocida B:2 GDH7 in bovine aortic endothelial cells (BAEC).
- Comparing the invasive properties of P. multocida B:2 GDH7 with its parent strain and P. multocida B:2 JRMT12.
- Transforming P. multocida B:2 GDH7 with a dual reporter plasmid (pSRGM) to track intracellular bacterial trafficking.
- Visualizing intracellular movement using confocal laser scanning microscopy (CLSM).
Main Results:
- P. multocida B:2 GDH7 exhibited significantly higher adherence to and invasion of BAEC compared to its parent strain and P. multocida B:2 JRMT12 (p ≤ 0.05).
- The strain survived intracellularly within BAEC for 7 hours post-treatment, with a gradual decrease over time.
- Intracellular trafficking of P. multocida B:2 GDH7 was successfully visualized using the reporter plasmid and CLSM.
Conclusions:
- The P. multocida B:2 GDH7 strain's capacity to undergo bactofection is demonstrated.
- This bactofection capability suggests potential for P. multocida B:2 GDH7 to serve as a delivery vector for DNA vaccines.
- The study supports the future use of P. multocida B:2 GDH7 for developing multivalent vaccines offering protection against HS in cattle and buffaloes.
