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Related Experiment Video

Updated: Feb 28, 2026

Author Spotlight: Developing a Microfluidic Lung-on-Chip Model for In-Depth Study of Human Immune Response and Infection Mechanisms

Author Spotlight: Developing a Microfluidic Lung-on-Chip Model for In-Depth Study of Human Immune Response and Infection Mechanisms

Published on: May 31, 2024

Human Lung Small Airway-on-a-Chip Protocol.

Kambez H Benam¹, Marc Mazur^1,2, Youngjae Choe¹

¹Wyss Institute for Biologically Inspired Engineering, Center for Life Science Building, 5th Floor, 3 Blackfan Circle, Boston, MA, 02115, USA.

Methods in Molecular Biology (Clifton, N.J.)

|June 22, 2017

Summary

This summary is machine-generated.

This study introduces a novel "small airway-on-a-chip" device that mimics human lung physiology. This organ-on-a-chip technology enables in vitro modeling of complex inflammatory responses in healthy and diseased lungs.

Keywords:

Lung pathophysiology Microfluidics Microphysiological System Organ-on-a-chip Small Airway Tissue microengineering

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Last Updated: Feb 28, 2026

Author Spotlight: Developing a Microfluidic Lung-on-Chip Model for In-Depth Study of Human Immune Response and Infection Mechanisms

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Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Published on: March 23, 2022

Area of Science:

Biotechnology
Microfluidics
Respiratory Medicine

Background:

Organs-on-chips are advanced microfluidic cell culture devices.
They replicate organ-level physiology in vitro using microchannels lined with living cells.
These devices recreate tissue interfaces, microenvironments, and vascular perfusion.

Purpose of the Study:

To describe the fabrication, culture, and operation of a human lung "small airway-on-a-chip" device.
To model complex and dynamic inflammatory responses in healthy and diseased lungs.
To provide a new microfluidic tool for in vitro lung research.

Main Methods:

Microengineering to fabricate a multilayered microfluidic device with two parallel elastomeric microchannels separated by a porous membrane.
Co-culture of primary human airway bronchiolar epithelial cells and lung microvascular endothelial cells on the membrane.
Flowing culture medium with neutrophils through the microvascular channel for real-time leukocyte analysis.

Main Results:

Successful fabrication of the microfluidic device in under 1 day.
Establishment of a mucociliated epithelium-endothelium interface over 4-6 weeks.
Real-time analysis of neutrophil capture and recruitment by endothelium under physiological shear.

Conclusions:

The "small airway-on-a-chip" is a novel microfluidic tool for in vitro lung modeling.
It effectively recapitulates key aspects of lung physiology and inflammatory responses.
This technology offers a new platform for studying respiratory diseases.