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Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus
Published on: June 15, 2017
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Using a Whole-mount Immunohistochemical Method to Study the Innervation of the Biliary Tract in Suncus murinus
1Department of Frontier Health Sciences, Graduate School of Human Health Sciences, Tokyo Metropolitan University.
Journal of Visualized Experiments : Jove
|June 28, 2017
Summary
This study details a whole-mount immunohistochemistry method to visualize nerve fibers in the biliary tract of Suncus murinus using neurofilament protein antibody. The protocol is adaptable for studying innervation in various organs and with other neuronal markers.
Area of Science:
- Neuroscience
- Histology
- Immunohistochemistry
Background:
- Understanding the innervation of visceral organs is crucial for physiological and pathological studies.
- Whole-mount immunohistochemistry offers advantages for visualizing complex neural networks in situ.
- Previous methods required adaptation for specific organ systems.
Purpose of the Study:
- To provide a detailed protocol for whole-mount immunohistochemistry of the biliary tract.
- To optimize the staining of neurofilament protein in Suncus murinus.
- To establish a versatile method for studying visceral organ innervation.
Main Methods:
- Dissection and fixation of Suncus murinus specimens.
- Enzymatic treatment and endogenous peroxidase inactivation.
- Incubation with primary (anti-neurofilament protein) and secondary antibodies, followed by DAB substrate reaction.
Main Results:
- Successful visualization of biliary tract innervation using neurofilament protein antibody.
- Demonstration of a robust whole-mount immunohistochemistry protocol.
- The method is applicable to other visceral organs and neuronal markers with optimization.
Conclusions:
- The described method provides a detailed and effective approach for whole-mount immunohistochemistry of visceral organ innervation.
- This protocol facilitates the study of neural pathways in the biliary system and beyond.
- The adaptability of the method allows for broader applications in neuroscience research.
