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Transgenic Plants

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Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
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Related Experiment Video

Updated: Feb 27, 2026

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Multiple T-DNA Delivery to Plants Using Novel Mini Binary Vectors with Compatible Replication Origins.

Fabio Pasin¹, Leonor C Bedoya¹, Joan Miquel Bernabé-Orts²

¹Centro Nacional de Biotecnología (CNB-CSIC) , Darwin 3, 28049 Madrid, Spain.

ACS Synthetic Biology

|June 29, 2017

Summary

New pLX vectors streamline plant genetic engineering. These mini binary T-DNA plasmids simplify multigene delivery using Agrobacterium-mediated transformation, advancing plant biotechnology.

Keywords:

Agrobacterium tumefaciens Potyviridae Ugandan cassava brown streak virus binary T-DNA vector ethanol-inducible switch multigene transfer pBBR1 plant biotechnology

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Area of Science:

Plant biotechnology
Molecular biology
Genetic engineering

Background:

Agrobacterium-mediated transformation is a key method for plant genetic modification.
Efficient DNA construct assembly into binary T-DNA vectors is crucial for plant gene delivery.
Existing vectors often require specific replication origins for bacterial maintenance.

Purpose of the Study:

To introduce pLX vectors, a novel set of mini binary T-DNA plasmids.
To enable efficient DNA assembly using Type IIS restriction endonuclease and overlap methods.
To facilitate multigene delivery for advanced plant engineering.

Main Methods:

Development of pLX vectors incorporating replicons from compatible broad host range plasmids.
Utilizing Type IIS restriction endonuclease and overlap-based assembly techniques.
Implementing a two-plasmid/one-Agrobacterium strain strategy with pBBR1- and RK2-based pLX vectors.

Main Results:

pLX vectors are mini binary T-DNA plasmids designed for streamlined assembly.
The vectors support compatible broad host range replication origins.
Simultaneous use of different pLX vector types enabled efficient multigene delivery to plants.

Conclusions:

pLX vectors simplify routine plant transformations and targeted mutagenesis.
These vectors are valuable tools for characterizing complex genetic parts and circuits.
Adoption of pLX vectors will accelerate advancements in plant biotechnology.