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Related Experiment Video

Updated: Feb 27, 2026

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

New DRB complexes for new DRB functions in plants.

Thomas Montavon¹, Yerim Kwon¹, Aude Zimmermann¹

¹a Université de Strasbourg, CNRS, IBMP UPR , Strasbourg , France.

|July 1, 2017

Summary

Double-stranded RNA binding (DRB) proteins can inhibit Dicer-like (DCL) processing by sequestering endogenous inverted-repeat dsRNAs. This challenges the view of DRBs as solely promoting small RNA production in plants.

Keywords:

Arabidopsis RNA silencing dicer double-stranded RNA binding protein siRNA

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A Method for Characterizing Embryogenesis in Arabidopsis

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Area of Science:

Molecular Biology
Plant Science
Genetics

Background:

Double-stranded RNA binding (DRB) proteins typically act as cofactors for Dicer or Dicer-like (DCL) proteins.
These proteins are crucial for the efficient and precise production of small RNAs, which guide RNA silencing processes.
Small RNAs are essential for gene regulation in both plants and animals.

Purpose of the Study:

To characterize a novel clade of DRB proteins in Arabidopsis.
To investigate the inhibitory role of these DRB proteins on DCL processing.
To explore the potential biological function of endoIR-siRNAs.

Main Methods:

Characterization of new DRB proteins in Arabidopsis.
Analysis of DRB protein interactions with dsRNA precursors.
Investigating the impact of DRB proteins on small RNA biogenesis.

Main Results:

A new clade of DRB proteins in Arabidopsis acts as potent inhibitors of DCL processing.
These DRBs sequester endogenous inverted-repeat (endoIR) dsRNAs.
This sequestration selectively prevents the production of endoIR-siRNAs.

Conclusions:

DRB proteins can function as inhibitors, not just promoters, of DCL activity.
The sequestration mechanism highlights a novel regulatory pathway in small RNA biogenesis.
The findings support a potential biological role for plant endoIR-siRNAs, though further research is needed.