Development of a conductivity-based photothermal absorbance detection microchip using polyelectrolytic gel
Honggu Chun1, Patty J Dennis2, Erin R Ferguson Welch2
1Department of Chemistry, University of North Carolina at Chapel Hill, Chapman Hall, CB#3216, Chapel Hill, NC 27599, United States; Department of Biomedical Engineering, Korea University, Hana Science Hall 466, Seoul, 02841, Republic of Korea.
Journal of Chromatography. A
|July 3, 2017
Summary
Polyelectrolytic gel electrodes (PGEs) enable sensitive microfluidic photothermal absorbance detection. This system measures conductivity changes for precise analyte quantification and separation.
Area of Science:
- Electrochemistry
- Analytical Chemistry
- Microfluidics
Background:
- Polyelectrolytic gel electrodes (PGEs) offer robust conductivity measurements.
- Microfluidic systems require sensitive detection methods for analytes.
Purpose of the Study:
- To develop and apply PGEs in a microfluidic photothermal absorbance detection system.
- To investigate the performance of 2-electrode and 3-electrode configurations.
Main Methods:
- Utilized PGEs for direct contact conductivity measurements.
- Employed photothermal absorbance detection with varying experimental parameters.
- Investigated both DC and AC voltage systems.
Main Results:
- Achieved limits of detection of 500 nM (3-electrode) and 0.55 nM (2-electrode) for DABSYL-tagged glucosamine.
- Demonstrated electrokinetic separation of a multi-analyte mixture.
- PGEs showed stability under high electric fields.
Conclusions:
- PGEs are effective for microfluidic photothermal absorbance detection.
- The developed system offers high sensitivity and separation capabilities.
- PGEs provide a stable platform for conductivity-based sensing.
