The subcellular distribution of DL-[3H]2-amino-4-phosphonobutyrate binding sites in the rat brain

The subcellular distribution of DL-[3H]2-amino-4-phosphonobutyrate binding sites in rat brain was examined. Binding sites for this radioligand, which selectively labels a chloride/calcium-dependent subpopulation of glutamate binding sites, were highly concentrated in synaptic membrane fractions. This is wholly consistent with the proposed involvement of this site in physiological responses.