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Cannabinoid-Induced Tetrad in Mice.

Mathilde Metna-Laurent1, Miguel Mondésir2,3, Agnès Grel2,3

  • 1Aelis Farma, Neurocentre Magendie, Bordeaux, France.

Current Protocols in Neuroscience
|July 6, 2017
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Summary
This summary is machine-generated.

The cannabinoid-induced tetrad model in mice reliably identifies central type-1 cannabinoid (CB1) receptor agonists. This preclinical model uses delta-9-tetrahydrocannabinol (THC) to induce hypolocomotion, hypothermia, catalepsy, and analgesia.

Keywords:
CB1 receptorscannabinoidmicetetrad

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Area of Science:

  • Pharmacology
  • Neuroscience
  • Preclinical Research

Background:

  • The cannabinoid-induced tetrad is a standard preclinical model for assessing central type-1 cannabinoid (CB1) receptor agonism in rodents.
  • The tetrad is defined by four key phenotypes: hypolocomotion, hypothermia, catalepsy, and analgesia.
  • These phenotypes are induced by acute administration of CB1 agonists, with delta-9-tetrahydrocannabinol (THC) serving as the prototypic example.

Purpose of the Study:

  • To describe a standardized protocol for inducing the tetrad phenotypes in mice using THC as a reference cannabinoid.
  • To present typical results demonstrating the dose-dependent effects of THC across different mouse strains.
  • To illustrate the impact of the CB1 antagonist rimonabant within this model.

Main Methods:

  • Administration of delta-9-tetrahydrocannabinol (THC) to mice to induce tetrad phenotypes.
  • Observation and measurement of four key behavioral and physiological responses: locomotion, body temperature, catalepsy, and analgesia.
  • Utilizing a CB1 antagonist (rimonabant) to validate the specificity of the observed effects.

Main Results:

  • THC administration reliably induced the four tetrad phenotypes in a dose-dependent manner.
  • Variations in THC's effects were observed across different mouse strains.
  • The CB1 antagonist rimonabant demonstrated its ability to block THC-induced tetrad effects.

Conclusions:

  • The described tetrad protocol provides a robust method for evaluating CB1 receptor activity in vivo.
  • This model is well-suited for the discovery and characterization of novel CB1 receptor-acting compounds.
  • The protocol's reproducibility and sensitivity make it valuable for preclinical drug development.