Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electrophoresis: Overview01:20

Electrophoresis: Overview

4.4K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
4.4K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

1.5K
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
1.5K
Capillary Electrophoresis: Instrumentation01:20

Capillary Electrophoresis: Instrumentation

1.3K
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
1.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Electrokinetic separation of bacteriophage ϕKZ from bacterial cells.

The Analyst·2026
Same author

Electrophoresis: An Effective Viability Assessment Method across Cell Domains.

Analytical chemistry·2026
Same author

Nonantiperiodic Nonlinear Electrophoresis of Colloidal Particles.

Analytical chemistry·2025
Same author

Fifty Years of Nonlinear Electrophoresis.

Electrophoresis·2025
Same author

Predicting the retention time of microparticles in electrokinetic migration.

The Analyst·2025
Same author

Tuning the Migration Order in Electrokinetic Separations of <i>Saccharomyces cerevisiae</i> Cells.

Analytical chemistry·2025

Related Experiment Video

Updated: Feb 27, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.7K

Exploiting Particle Mutual Interactions To Enable Challenging Dielectrophoretic Processes.

Mario A Saucedo-Espinosa1, Blanca H Lapizco-Encinas1

  • 1Microscale Bioseparations Laboratory, Rochester Institute of Technology , Rochester, New York 14623, United States.

Analytical Chemistry
|July 8, 2017
PubMed
Summary

Filler particles enhance insulator-based dielectrophoresis (iDEP) for improved yeast cell enrichment. Small filler particles, at low concentrations, significantly boost iDEP efficiency, achieving high enrichment factors with minimal voltage.

More Related Videos

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

28.1K
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

9.6K

Related Experiment Videos

Last Updated: Feb 27, 2026

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
10:38

Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis

Published on: September 3, 2013

16.7K
Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow
09:45

Separating Beads and Cells in Multi-channel Microfluidic Devices Using Dielectrophoresis and Laminar Flow

Published on: February 4, 2011

28.1K
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

9.6K

Area of Science:

  • Biophysics
  • Microfluidics
  • Electrokinetics

Background:

  • Dielectrophoresis (DEP) describes particle movement in nonuniform electric fields.
  • Insulator-based DEP (iDEP) utilizes insulating structures in microchannels to create these fields.
  • Understanding particle interactions is crucial for optimizing iDEP applications.

Purpose of the Study:

  • To experimentally characterize electrokinetic (EK) and DEP velocities of particles in iDEP.
  • To investigate the effect of filler particles on particle behavior and iDEP performance.
  • To enhance the enrichment of low-abundance yeast cells using iDEP.

Main Methods:

  • Streak-based particle velocimetry was employed in a tapered microchannel.
  • Various sizes and volume fractions of filler particles were introduced into the suspending medium.
  • DC potentials were applied to induce and measure particle motion.

Main Results:

  • Filler particles induced electric field distortions and altered particle behaviors through mutual interactions.
  • The smallest filler particles (500 nm) at volume fractions of ~1x10^-5 v/v significantly aided yeast cell enrichment.
  • Enrichment factors of approximately 115 were achieved using potentials as low as 500 V.

Conclusions:

  • Filler particles can be strategically used to improve iDEP performance.
  • Exploiting particle-particle interactions with small fillers offers a viable method for low-abundance cell enrichment.
  • This approach demonstrates efficient yeast cell enrichment in iDEP systems.