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Visualization and Analysis of mRNA Molecules Using Fluorescence In Situ Hybridization in Saccharomyces cerevisiae
Published on: June 14, 2013
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Saccharomyces cerevisiae MSA1 mRNA has a sequence for localization at the bud tip
Tomoko Takeuchi-Andoh1, Yukiko Hayano-Oshiro2, Emi Nishiyoshi1
1a Junior College Division , Otsuma Women's University , Tokyo , Japan.
Bioscience, Biotechnology, and Biochemistry
|July 12, 2017
Summary
This study reveals that MSA1 mRNA localizes to the bud tip, a process dependent on SHE2. This mRNA localization influences Msa1p protein levels, suggesting a novel regulatory mechanism for Msa1p.
Area of Science:
- Cell biology
- Molecular genetics
- Budding yeast research
Background:
- MSA1 mRNA encodes Msa1p, a protein regulating G1 phase gene transcription.
- Msa1p abundance and localization are cell cycle-dependent.
- CDK1 phosphorylation controls Msa1p nuclear entry.
Purpose of the Study:
- To investigate the subcellular localization of MSA1 mRNA.
- To identify the regulatory elements and factors involved in MSA1 mRNA localization.
- To understand the functional implications of MSA1 mRNA localization on Msa1p regulation.
Main Methods:
- Screening for localized mRNAs using a Tag-GFP system.
- Fragmentation analysis to determine the localization sequence of MSA1 mRNA.
- Investigating the role of SHE2 in MSA1 mRNA localization and Msa1p levels.
Main Results:
- MSA1 mRNA was identified as a bud tip-localized mRNA.
- A specific sequence of approximately 145 bases mediates bud tip localization.
- Endogenous MSA1 mRNA localization at the bud tip requires SHE2.
- SHE2 also impacts Msa1p levels in cells with constitutive MSA1 mRNA expression.
Conclusions:
- MSA1 mRNA exhibits specific localization to the bud tip in yeast.
- The SHE2 protein is crucial for the bud tip localization of MSA1 mRNA.
- Localized control of MSA1 mRNA represents a novel regulatory mechanism for Msa1p.
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