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[The indirect solid-phase method of immunoenzyme analysis, its accuracy and sources of error]
Zhurnal Mikrobiologii, Epidemiologii I Immunobiologii
|March 1, 1986
Summary
The enzyme-linked immunosorbent assay (ELISA) shows low accuracy due to titration and plate variations. Instruments like dispensers and photometers also impact results, with Soviet-made plates causing significant errors.
Area of Science:
- Biochemical assays
- Immunodiagnostics
Background:
- Enzyme-linked immunosorbent assay (ELISA) is a widely used diagnostic tool.
- Ensuring the accuracy of ELISA is crucial for reliable diagnostic outcomes.
Purpose of the Study:
- To investigate the factors affecting the accuracy of the enzyme-linked immunosorbent assay (ELISA).
- To identify the primary sources of error in ELISA procedures.
Main Methods:
- Evaluation of instrument variability, including dispensers, photometers, and microplates.
- Analysis of titration procedures and their impact on assay performance.
- Assessment of adsorption and optical properties of different microplates.
Main Results:
- The overall accuracy of the enzyme-linked immunosorbent assay (ELISA) was found to be low.
- Titration procedures were identified as a major source of error.
- Significant variations in adsorption and optical properties among microplates were observed.
- Microplates manufactured in the USSR exhibited greater errors compared to others.
Conclusions:
- The accuracy of ELISA is significantly influenced by procedural and material factors.
- Standardization of titration methods and microplate characteristics is essential for improving ELISA reliability.
- Careful selection of microplates is recommended to minimize diagnostic errors.