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Introducing a Gene Knockout Directly Into the Amastigote Stage of Trypanosoma cruzi Using the CRISPR/Cas9 System
Published on: July 31, 2019
A targeted delivery strategy for the development of potent trypanocides
Heeren M Gordhan1, Jillian E Milanes2, Yijian Qiu2
1Department of Chemistry, Clemson University, Clemson, SC, USA. dwhiteh@clemson.edu.
Abstract:
A new drug delivery strategy was investigated for the development of potent anti-parasitic compounds against Trypanosoma brucei, the causative agent of African sleeping sickness. Thus, potent in vitro hexokinase inhibitors were rendered cytotoxic by appending a tripeptide peroxosomal targeting sequence that facilitated delivery of the molecular cargo to the appropriate organelle in the parasite.
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