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Diffraction tomography with Fourier ptychography.

Roarke Horstmeyer1,2, Jaebum Chung1, Xiaoze Ou1

  • 1Department of Electrical Engineering, California Institute of Technology, Pasadena, California 91125, USA.

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|July 25, 2017
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Summary
This summary is machine-generated.

Fourier ptychographic tomography (FPT) enables 3D refractive index imaging using a standard microscope and LEDs. This technique reconstructs complex sample structures without moving parts, offering new possibilities for biological imaging.

Keywords:
(110.6955) Tomographic imaging(180.6900) Three-dimensional microscopy

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Area of Science:

  • Optics
  • Biomedical Imaging
  • Computational Microscopy

Background:

  • Imaging the complex refractive index is crucial for analyzing transparent biological samples.
  • Existing methods often require specialized hardware or lack quantitative volumetric measurements.
  • Fourier ptychography and diffraction tomography offer complementary approaches to imaging.

Purpose of the Study:

  • To present a novel technique, Fourier ptychographic tomography (FPT), for 3D refractive index imaging.
  • To demonstrate FPT's capability using a standard microscope and LED illumination.
  • To enable quantitative volumetric imaging of transparent samples without interferometry.

Main Methods:

  • Capturing intensity-only images of a sample under angularly varying LED illumination.
  • Applying principles of ptychography and diffraction tomography for computational reconstruction.
  • Developing a 3D imaging approach using a standard microscope setup.

Main Results:

  • Achieved lateral resolution of 0.39 μm and axial resolution of 3.7 μm.
  • Successfully imaged a total depth of 110 μm with high resolution.
  • Quantitatively measured volumetric refractive index of transparent sample features.

Conclusions:

  • Fourier ptychographic tomography (FPT) provides a hardware-efficient method for 3D refractive index imaging.
  • The technique overcomes limitations of existing methods for transparent samples.
  • Potential applications in pathology and developmental biology due to wide field-of-view reconstructions of thick specimens.