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Updated: Feb 26, 2026

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Published on: April 21, 2022
Statistical approach for ATR-FTIR screening of semen in sexual evidence
Inês Gregório1, Félix Zapata1, Mercedes Torre1
1Department of Analytical Chemistry, Physical Chemistry and Chemical Engineering and Institute of Research in Police Sciences (IUICP), University of Alcalá, Ctra. Madrid-Barcelona km 33.600, 28871 Alcalá de Henares, Madrid, Spain.
Abstract:
Genetic identification has revolutionized the Forensic Sciences, especially in sexual aggression cases. For the successful extraction of the genetic information of a criminal, a crucial step is the prior detection of bodily fluids on evidence. In this article, a method for non-destructive screening of semen samples is reported. Using chemometric tools, bodily fluids can be detected and differentiated without damaging the sample, by correlating the infrared spectra of sexual evidence with previously recorded spectra from undamaged stains of individual bodily fluids. In modern hospitals/laboratories, the proposed method would not require additional equipment/material nor specialized personnel. Furthermore, the method provides qualitative and reliable results, without requiring human interpretation. Therefore, the proposed method opens a door for a low-cost, fully automated and efficient system for non-destructive screening of semen, which could be easily and massively implemented.
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