Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

The use of supplemental oxygen during cardiopulmonary resuscitation - a systematic review of the literature.

Resuscitation plus·2026

Same author

Topographical memory in transient epileptic amnesia.

Epilepsy & behavior : E&B·2026

Same author

Donor-derived cell-free DNA in chronic lung allograft dysfunction phenotypes: a pilot study.

Frontiers in transplantation·2025

Same author

Preparation of large biological samples for high-resolution, hierarchical, synchrotron phase-contrast tomography with multimodal imaging compatibility.

Nature protocols·2023

Same author

Trends in mortality from aortic dissection analyzed from the World Health Organization Mortality Database from 2000 to 2017.

International journal of cardiology·2022

Same author

The Eruption of the Permanent Teeth.

The Dental register·2021

Search research articles

Related Experiment Video

Updated: Feb 26, 2026

Low-Dose Gamma Radiation Sterilization for Decellularized Tracheal Grafts

Low-Dose Gamma Radiation Sterilization for Decellularized Tracheal Grafts

Published on: April 14, 2023

An optimized non-destructive protocol for testing mechanical properties in decellularized rabbit trachea.

M Den Hondt¹, B M Vanaudenaerde², E F Maughan³

¹Department of Plastic & Reconstructive Surgery, University Hospitals Leuven, Herestraat 49, 3000 Leuven, Belgium.

Acta Biomaterialia

|July 26, 2017

Summary

This summary is machine-generated.

A novel non-destructive biomechanical testing method using microCT was developed for tissue-engineered trachea. This method revealed functional integrity loss in decellularized scaffolds not detected by traditional analyses, crucial for clinical applications.

Keywords:

Biomechanical properties Decellularization Scaffold Tissue engineering Trachea transplantation

More Related Videos

Investigating Stress-relaxation and Failure Responses in the Trachea

Investigating Stress-relaxation and Failure Responses in the Trachea

Published on: October 18, 2022

Design of a Biocompatible Drug-Eluting Tracheal Stent in Mice with Laryngotracheal Stenosis

Design of a Biocompatible Drug-Eluting Tracheal Stent in Mice with Laryngotracheal Stenosis

Published on: January 21, 2020

Related Experiment Videos

Last Updated: Feb 26, 2026

Low-Dose Gamma Radiation Sterilization for Decellularized Tracheal Grafts

Low-Dose Gamma Radiation Sterilization for Decellularized Tracheal Grafts

Published on: April 14, 2023

Investigating Stress-relaxation and Failure Responses in the Trachea

Investigating Stress-relaxation and Failure Responses in the Trachea

Published on: October 18, 2022

Design of a Biocompatible Drug-Eluting Tracheal Stent in Mice with Laryngotracheal Stenosis

Design of a Biocompatible Drug-Eluting Tracheal Stent in Mice with Laryngotracheal Stenosis

Published on: January 21, 2020

Area of Science:

Biomaterials Science
Regenerative Medicine
Tissue Engineering

Background:

Tissue-engineered tracheal transplantation requires scaffolds with specific mechanical properties and biocompatibility.
Current methods for assessing scaffold mechanical properties are destructive and do not evaluate to physiological limits.
Decellularization is a key strategy for generating tracheal scaffolds, but preserving mechanical integrity is critical.

Purpose of the Study:

To develop and evaluate a novel, non-destructive method for biomechanical testing of decellularized tracheae.
To assess the impact of detergent-enzymatic decellularization cycles on tracheal scaffold integrity.
To validate the developed method by comparing its findings with quantitative, qualitative, and in vivo analyses.

Main Methods:

Rabbit tracheae underwent varying cycles (2, 4, 8) of detergent-enzymatic decellularization.
Biomechanical properties were assessed non-destructively using microCT to measure luminal volume during inflation/deflation.
Quantitative analysis included DNA, glycosaminoglycan, and collagen content; qualitative analysis assessed scaffold integration in vivo.

Main Results:

Tracheae decellularized for 2 and 4 cycles maintained mechanical properties similar to native trachea.
Eight-cycle decellularization led to significant collapsibility and inadequate in vivo integration.
The microCT-based method detected loss of functional integrity with increased decellularization cycles, which was not apparent through conventional analyses.

Conclusions:

Limited detergent-enzymatic decellularization can remove immunogenic material without compromising mechanical stability.
Progressive decellularization cycles compromise functional integrity, leading to mechanical instability and poor in vivo performance.
Non-destructive functional evaluation, such as microCT-based biomechanical testing, is essential for screening tracheal scaffolds before clinical use.