Related Experiment Video
Updated: Feb 25, 2026
Author Spotlight: Exploring Plant-Microbe Interactions Through Root Exudates in a Novel Growth System
Published on: November 17, 2023
Comparison of sodium hypochlorite extrusion by five irrigation systems using an artificial root socket model and a
Adham A Azim1,2, Hacer Aksel1,3, M Margaret Jefferson1
1Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, 875 Union Avenue, Laboratory, Cancer Research Building, 19 S. Manassas St. Lab Rm 255, office 256, Memphis, TN, 38163, USA.
Objectives:
This is to compare the volumes of irrigant apically extruded by five irrigation systems in an artificial socket model simulating clinical conditions.
Materials And Methods:
Twenty extracted human single-rooted teeth were enlarged to size 40/04 and then embedded in silicone impression material. The root canal space was irrigated with nominal 3% sodium hypochlorite (NaOCl) using standard needle irrigation (SNI) with a 30-gauge notched needle, EndoActivator (EA), XP Endo Finisher (XP Endo), EndoVac (EV), and photon-induced photoacoustic streaming (PIPS). Extruded NaOCl was collected, reacted with taurine to form taurine-monochloramine, and absorbance of taurine-monochloramine was measured at 252 nm using a spectrophotometer. The five irrigation systems were compared with repeated measures ANOVA and pairwise comparisons.
Results:
The EV group had very low extrusion (mean ± SD = 0.12 ± 0.2 μL) and differed significantly from the other four groups (P ≤ 0.001). Larger volumes of irrigant were extruded in the other irrigation groups. There were no significant differences in the extruded volumes among the SNI (7.4 ± 3.4 μL), EA (7.0 ± 6.1 μL), and XP Endo (7.8 ± 4.1 μL) groups (P = 1). The PIPS group had the highest mean extruded volume (12.9 ± 6.8 μL) and differed significantly from SNI (P = 0.030), EV (P < 0.0005), and EA (P = 0.02), but not XP Endo (P = 0.154).
Conclusion:
Under the in vitro conditions of this study, irrigant extrusion appears unavoidable unless negative pressure irrigation such as EV is used. PIPS extrudes more irrigant than other systems, while SNI, EA, and XP Endo extrude similar volumes of irrigant.
Clinical Relevance:
The findings help clinicians select the optimal irrigation system to avoid irrigant extrusion.
